PDBsum entry 9cbl

Signaling protein

PDB id: 9cbl

Contents

Protein chains

211 a.a.

338 a.a.

60 a.a.

256 a.a.

Ligands

ALE

PDB id:

9cbl

Name:

Signaling protein

Title:

Cryo-em structure of epinephrine-bound alpha-2a-adrenergic receptor in complex with heterotrimeric gi-protein

Structure:

Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein. Engineered: yes. Mutation: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1.

Source:

Rattus norvegicus. Norway rat. Organism_taxid: 10116. Gene: gnai1, gnai-1. Expressed in: escherichia coli. Expression_system_taxid: 562. Bos taurus. Cattle. Organism_taxid: 9913.

Authors:

J.S.Lou,M.Su,J.Wang,H.N.Do,Y.Miao,X.Y.Huang

Key ref:

J.S.Lou et al. Distinct binding conformations of epinephrine with al and beta-Adrenergic receptors.. Exp mol med, . PubMed id: 39218975

Date:

19-Jun-24 Release date: 11-Sep-24

Protein chain

P10824  (GNAI1_RAT) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Rattus norvegicus

Seq: 354 a.a.

Struc: 211 a.a.*

Protein chain

P62871  (GBB1_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Bos taurus

Seq: 340 a.a.

Struc: 338 a.a.

Protein chain

P63212  (GBG2_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Bos taurus

Seq: 71 a.a.

Struc: 60 a.a.

Protein chain

P00720  (ENLYS_BPT4) -  Endolysin from Enterobacteria phage T4

Seq: 164 a.a.

Struc: 256 a.a.*

Protein chain

P08913  (ADA2A_HUMAN) -  Alpha-2A adrenergic receptor from Homo sapiens

Seq: 465 a.a.

Struc: 256 a.a.

* PDB and UniProt seqs differ at 164 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain R: E.C.3.2.1.17 - lysozyme.
• Reaction: Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.