PDBsum entry 8wpe

Viral protein

PDB id: 8wpe

Contents

Protein chains

1006 a.a.

426 a.a.

218 a.a.

69 a.a.

57 a.a.

65 a.a.

DNA/RNA

Ligands

D3T

Metals

_MG

PDB id:

8wpe

Name:

Viral protein

Title:

Structure of monkeypox virus polymerase complex f8-a22-e4-h5 (tag-free a22) with exogenous DNA

Structure:

DNA polymerase. Chain: a. Engineered: yes. Other_details: genbank: urk20494.1. A22r DNA polymerase processivity factor. Chain: b. Engineered: yes. Other_details: genbank: urk20570.1. E4r uracil-DNA glycosylase, DNA polymerase processivity

Source:

Monkeypox virus. Organism_taxid: 10244. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293f. Synthetic: yes. Synthetic construct. Organism_taxid: 32630. Organism_taxid: 32630

Authors:

X.Wang,N.Li,N.Gao

Key ref:

X.Wang et al. (2023). Structural insights into the assembly and mechanism o monkeypox virus DNA polymerase complex f8-A22-E4-H5. Mol cell, 83, . PubMed id: 37995690

Date:

10-Oct-23 Release date: 29-Nov-23

Protein chain

A0A7H0DN44  (DPOL_MONPV) -  DNA polymerase from Monkeypox virus

Seq: 1006 a.a.

Struc: 1006 a.a.*

Protein chain

A0A7H0DNC0  (PG148_MONPV) -  DNA polymerase processivity factor component OPG148 from Monkeypox virus

Seq: 426 a.a.

Struc: 426 a.a.

Protein chain

M1LL92  (UNG_MONPV) -  Uracil-DNA glycosylase from Monkeypox virus

Seq: 218 a.a.

Struc: 218 a.a.

Protein chain

A0A7H0DN82  (PG110_MONPV) -  Late transcription elongation factor OPG110 from Monkeypox virus

Seq: 210 a.a.

Struc: 69 a.a.

Protein chains

A0A7H0DN82  (PG110_MONPV) -  Late transcription elongation factor OPG110 from Monkeypox virus

Seq: 210 a.a.

Struc: 57 a.a.

Protein chain

A0A7H0DN82  (PG110_MONPV) -  Late transcription elongation factor OPG110 from Monkeypox virus

Seq: 210 a.a.

Struc: 65 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

C-G-C-G-G-G-A-G-C-T-A-T-G-A-C-C-A-T-G-A-T-T-A-C-G-A-A-T-T-G-C 31 bases

G-A-A-T-A-A-G-C-A-A-T-T-C-G-T-A-A-T-C-A-T-G-G-T-C-A-T-A-G-C-T-C-C-C-G-C-G 37 bases

Enzyme reactions

• Enzyme class 2: Chain A: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 3: Chain C: E.C.3.2.2.27 - uracil-DNA glycosylase.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site