PDBsum entry 8v6g

Replication, transferase

PDB id: 8v6g

Contents

Protein chains

1050 a.a.

431 a.a.

435 a.a.

381 a.a.

DNA/RNA

Ligands

DGT

SF4

Metals

_MG ×2

_ZN ×3

PDB id:

8v6g

Name:

Replication, transferase

Title:

DNA initiation complex (configuration 1) of xenopus laevis DNA polymerase alpha-primase

Structure:

DNA polymerase alpha catalytic subunit. Chain: a. Fragment: unp residues 335-1458. Synonym: DNA polymerase alpha catalytic subunit p180. Engineered: yes. DNA polymerase alpha subunit b. Chain: b. Engineered: yes. DNA primase large subunit.

Source:

Xenopus laevis. African clawed frog. Organism_taxid: 8355. Gene: pola1, pola. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: pola2.L. Gene: prim2.S. Expressed in: escherichia coli.

Authors:

E.A.Mullins,C.L.Durie,M.D.Ohi,W.J.Chazin,B.F.Eichman

Key ref:

E.A.Mullins et al. A mechanistic model of primer synthesis from catalyti structures of DNA polymerase alpha-Primase. To be published, . PubMed id: 38491139

Date:

01-Dec-23 Release date: 20-Dec-23

Protein chain

Q9DE46  (DPOLA_XENLA) -  DNA polymerase alpha catalytic subunit from Xenopus laevis

Seq: 1458 a.a.

Struc: 1050 a.a.

Protein chain

Q6DCZ1  (Q6DCZ1_XENLA) -  DNA polymerase alpha subunit B from Xenopus laevis

Seq: 598 a.a.

Struc: 431 a.a.

Protein chain

A0A1L8G3G3  (A0A1L8G3G3_XENLA) -  DNA primase large subunit from Xenopus laevis

Seq: 513 a.a.

Struc: 435 a.a.

Protein chain

Q800A4  (Q800A4_XENLA) -  DNA primase from Xenopus laevis

Seq: 420 a.a.

Struc: 381 a.a.

DNA/RNA chains

T-C-A-C-G-C-A-G-T-A-T-C-C-T-G-T 16 bases

GTP-G-A-U-A-C-U-G-DOC 9 bases

Enzyme reactions

• Enzyme class 1: Chain A: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate
• Enzyme class 2: Chains B, C: E.C.?
• Enzyme class 3: Chain D: E.C.2.7.7.- - ?????

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site