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UniProt functional annotation for Q8Y5E4 | ||
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| UniProt code: Q8Y5E4. |
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| Organism: | |
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria. |
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| Function: | |
Catalyzes the condensation of 2 ATP molecules into cyclic di- AMP (c-di-AMP) (PubMed:25605729). c-di-AMP is a signaling compound secreted into the host's cytosol where it triggers the cytosolic surveillance pathway (CSP), a host pathway of innate immunity characterized by expression of beta interferon (IFN-beta) and coregulated genes (PubMed:20508090). Expression of truncated proteins (missing first 80 or 100 residues) in E.coli leads to c-di-AMP synthesis (PubMed:25605729). {ECO:0000269|PubMed:20508090, ECO:0000269|PubMed:25605729}. |
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| Catalytic activity: | |
Reaction=2 ATP = 3',3'-c-di-AMP + 2 diphosphate; Xref=Rhea:RHEA:35655, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:71500; EC=2.7.7.85; Evidence={ECO:0000255|HAMAP-Rule:MF_01499}; |
| Cofactor: | |
Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:25605729}; Note=Optimal activity at 0.5-1 mM CoCl(2), also functions with Mn(2+), for a construct missing residues 1-80. {ECO:0000269|PubMed:25605729}; |
| Subunit: | |
Homodimer, required for active site formation (Probable). {ECO:0000305|PubMed:25605729}. |
| Subcellular location: | |
Cell membrane {ECO:0000255|HAMAP-Rule:MF_01499}; Multi-pass membrane protein {ECO:0000255|HAMAP-Rule:MF_01499}. |
| Disruption phenotype: | |
Essential, it cannot be deleted. {ECO:0000269|PubMed:20508090}. |
| Similarity: | |
Belongs to the adenylate cyclase family. DacA/CdaA subfamily. {ECO:0000255|HAMAP-Rule:MF_01499}. |
Annotations taken from UniProtKB at the EBI.