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UniProt functional annotation for O60942 | ||
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| UniProt code: O60942. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Bifunctional mRNA-capping enzyme exhibiting RNA 5'- triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus. {ECO:0000269|PubMed:21636784, ECO:0000269|PubMed:9473487, ECO:0000269|PubMed:9512541}. |
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| Catalytic activity: | |
Reaction=a 5'-end triphospho-(purine-ribonucleoside) in mRNA + H2O = a 5'-end diphospho-(purine-ribonucleoside) in mRNA + H(+) + phosphate; Xref=Rhea:RHEA:11008, Rhea:RHEA-COMP:13929, Rhea:RHEA-COMP:13942, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:43474, ChEBI:CHEBI:138276, ChEBI:CHEBI:138288; EC=3.1.3.33; |
| Catalytic activity: | |
Reaction=a 5'-end diphospho-ribonucleoside in mRNA + GTP + H(+) = a 5'- end (5'-triphosphoguanosine)-(ribonucleoside) in mRNA + diphosphate; Xref=Rhea:RHEA:67012, Rhea:RHEA-COMP:17165, Rhea:RHEA-COMP:17166, ChEBI:CHEBI:15378, ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:167616, ChEBI:CHEBI:167617; EC=2.7.7.50; |
| Subunit: | |
Interacts with POLR2A (via C-terminus); this enhances guanylyltransferase activity. Binds (via GTase domain) to the elongating phosphorylated form of RNA polymerase II; can form direct interactions with the phosphorylated POLR2A C-terminal domain and indirect interactions via bound RNA (By similarity). Interacts with SUPT5H and RNMT. Interacts with HIV-1 Tat. {ECO:0000250, ECO:0000269|PubMed:10421630, ECO:0000269|PubMed:9705270}. |
| Subunit: | |
(Microbial infection) Interacts with HIV-1 Tat. {ECO:0000269|PubMed:11278368}. |
| Subcellular location: | |
Nucleus {ECO:0000250}. |
| Tissue specificity: | |
Isoform 1 and isoform 4 (at a lesser extent) are expressed in cerebrum, cerebellum, thyroid, lung, heart, liver, kidney, spleen, large intestine, testis, skin and muscle. |
| Miscellaneous: | |
Isoform 2 to isoform 4 lack mRNA 5'-guanylyltransferase activity due to disruptions of the GTase domain. |
| Similarity: | |
In the N-terminal section; belongs to the non-receptor class of the protein-tyrosine phosphatase family. {ECO:0000305}. |
| Similarity: | |
In the C-terminal section; belongs to the eukaryotic GTase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.