PDBsum entry 8ieg

Nuclear protein

PDB id: 8ieg

Contents

Protein chains

98 a.a.

80 a.a.

109 a.a.

94 a.a.

64 a.a.

148 a.a.

DNA/RNA

Metals

_ZN ×4

PDB id:

8ieg

Name:

Nuclear protein

Title:

Bre1(mrbd-ring)/rad6-ub/nucleosome complex

Structure:

Histone h3.1. Chain: k, e. Synonym: histone h3/a,histone h3/b,histone h3/c,histone h3/d,histone h3/f,histone h3/h,histone h3/i,histone h3/j,histone h3/k,histone h3/l. Engineered: yes. Histone h4. Chain: l, f. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: h3c1, h3fa, hist1h3a, h3c2, h3fl, hist1h3b, h3c3, h3fc hist1h3c, h3c4, h3fb, hist1h3d, h3c6, h3fd, hist1h3e, h3c7, h3fi, hist1h3f, h3c8, h3fh, hist1h3g, h3c10, h3fk, hist1h3h, h3c11, h3ff, hist1h3i, h3c12, h3fj, hist1h3j. Expressed in: escherichia coli. Expression_system_taxid: 562.

Authors:

H.Ai,Z.Deng,M.Pan,L.Liu

Key ref:

Z.Deng et al. Mechanistic insights into nucleosomal h2b monoubiquit mediated by yeast bre1-Rad6 and its human homolog rnf20/rnf40-Hrad6a.. Mol cell, . PubMed id: 37633270

Date:

15-Feb-23 Release date: 06-Sep-23

Protein chains

P68431  (H31_HUMAN) -  Histone H3.1 from Homo sapiens

Seq: 136 a.a.

Struc: 98 a.a.

Protein chains

P62805  (H4_HUMAN) -  Histone H4 from Homo sapiens

Seq: 103 a.a.

Struc: 80 a.a.

Protein chains

P04908  (H2A1B_HUMAN) -  Histone H2A type 1-B/E from Homo sapiens

Seq: 130 a.a.

Struc: 109 a.a.

Protein chains

O60814  (H2B1K_HUMAN) -  Histone H2B type 1-K from Homo sapiens

Seq: 126 a.a.

Struc: 94 a.a.*

Protein chains

Q07457  (BRE1_YEAST) -  E3 ubiquitin-protein ligase BRE1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 700 a.a.

Struc: 64 a.a.

Protein chain

P06104  (UBC2_YEAST) -  Ubiquitin-conjugating enzyme E2 2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 172 a.a.

Struc: 148 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

DNA/RNA chains

A-C-A-G-G-A-T-G-T-A-T-A-T-A-T-C-T-G-A-C-A-C-G-T-G-C-C-T-G-G-A-G-A-C-T-A-G-G-G- 147 bases

C-T-G-G-A-G-A-A-T-C-C-C-G-G-T-G-C-C-G-A-G-G-C-C-G-C-T-C-A-A-T-T-G-G-T-C-G-T-A- 147 bases

Enzyme reactions

• Enzyme class 2: Chains A, B: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 3: Chain R: E.C.2.3.2.23 - E2 ubiquitin-conjugating enzyme.
• Reaction: S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L- cysteine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.