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UniProt functional annotation for P18014 | ||
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| UniProt code: P18014. |
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| Organism: | |
Shigella flexneri. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Shigella. |
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| Function: | |
E3 ubiquitin ligase effector protein that interferes with host's ubiquitination pathway (PubMed:30872533). Functions to alter host cell physiology and promote bacterial survival in host tissues (PubMed:30872533). Catalyzes ubiquitination of mouse Nlrp1b allele 1 protein, releasing the cleaved C-terminal part of Nlrp1b, which polymerizes and forms the Nlrp1b inflammasome followed by host cell pyroptosis (PubMed:30872533). {ECO:0000269|PubMed:30872533}. |
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| Catalytic activity: | |
Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L- cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.; EC=2.3.2.27; Evidence={ECO:0000269|PubMed:30872533}; |
| Subcellular location: | |
Secreted {ECO:0000250|UniProtKB:D0ZVG2}. Host cytoplasm {ECO:0000250|UniProtKB:D0ZVG2}. Note=Secreted via Mxi-Spa type III secretion system (TTSS), and delivered into the host cytoplasm. {ECO:0000250|UniProtKB:D0ZVG2}. |
| Domain: | |
The LRR (leucine-rich repeat) domain forms a slightly curved solenoid and may mediate interaction with target proteins. {ECO:0000250|UniProtKB:D0ZPH9}. |
| Ptm: | |
Ubiquitinated in the presence of host E1 ubiquitin-activating enzyme, E2 ubiquitin-conjugating enzyme and ubiquitin. {ECO:0000250|UniProtKB:D0ZPH9}. |
| Similarity: | |
Belongs to the LRR-containing bacterial E3 ligase family. {ECO:0000305}. |
| Sequence caution: | |
Sequence=AAA26526.1; Type=Frameshift; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.