PDBsum entry 8b4b

DNA binding protein

PDB id: 8b4b

Contents

Protein chains

110 a.a.

104 a.a.

DNA/RNA

Ligands

NH4 ×2

Metals

_CD ×24

Waters ×528

PDB id:

8b4b

Name:

DNA binding protein

Title:

Toxr bacterial transcriptional regulator bound to 19 bp ompu promoter DNA

Structure:

DNA (19-mer). Chain: l. Engineered: yes. DNA (19-mer). Chain: m. Engineered: yes. Cholera toxin transcriptional activator. Chain: w, x, y, z. Engineered: yes

Source:

Synthetic: yes. Vibrio cholerae. Organism_taxid: 666. Strain: atcc 39315 / el tor inaba n16961. Gene: toxr, vc_0984. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

1.75Å R-factor: 0.201 R-free: 0.235

Authors:

A.Canals,S.Pieretti,M.Muriel,N.El Yaman,M.Fabrega-Ferrer,R.Perez- Luque,E.S.Krukonis,M.Coll

Key ref:

A.Canals et al. (2023). Toxr activates the vibrio cholerae virulence genes by tethering DNA to the membrane through versatile bindi multiple sites.. Proc natl acad sci usa, 120, 78120. PubMed id: 37428913

Date:

20-Sep-22 Release date: 09-Aug-23

Protein chains

P15795  (TOXR_VIBCH) -  Cholera toxin transcriptional activator from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

Seq: 294 a.a.

Struc: 110 a.a.*

Protein chains

P15795  (TOXR_VIBCH) -  Cholera toxin transcriptional activator from Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961)

Seq: 294 a.a.

Struc: 104 a.a.*

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

DNA/RNA chains

C-A-T-A-T-C-A-T-T-T-T-A-C-T-A-A-C-T-G 19 bases

C-A-G-T-T-A-G-T-A-A-A-A-T-G-A-T-A-T-G 19 bases

Enzyme reactions

• Enzyme class: Chains W, X, Y, Z: E.C.?