PDBsum entry 8yh0

Signaling protein

PDB id: 8yh0

Contents

Protein chains

271 a.a.

211 a.a.

336 a.a.

53 a.a.

232 a.a.

Ligands

NEC

PDB id:

8yh0

Name:

Signaling protein

Title:

A3r-gi complex bound to neca

Structure:

Ha tag,adenosine receptor a3,lgbit,egfp chimera. Chain: r. Engineered: yes. Other_details: flag tag (dykddddk). Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2,guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a, g. Synonym: g gamma-i,adenylate cyclase-inhibiting g alpha protein. Engineered: yes.

Source:

Influenza a virus (a/victoria/3/1975(h3n2)), ovis aries. Sheep. Organism_taxid: 392809, 9940. Gene: ha, adora3. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293. Homo sapiens.

Authors:

H.S.Oshima,W.Shihoya,O.Nureki

Key ref:

H.S.Oshima et al. Structural insight into the agonist selectivity and structure-Based engineering of the adenosine a3 recep. To be published, . PubMed id: 39511145

Date:

27-Feb-24 Release date: 06-Nov-24

Protein chain

A0A5P9VSM6  (A0A5P9VSM6_HRSV) -  GFP from Human respiratory syncytial virus

Seq: 239 a.a.

Struc: 271 a.a.*

Protein chain

P03435  (HEMA_I75A3) -  Hemagglutinin from Influenza A virus (strain A/Victoria/3/1975 H3N2)

Seq: 567 a.a.

Struc: 271 a.a.*

Protein chain

W5QED6  (W5QED6_SHEEP) -  Adenosine receptor A3 from Ovis aries

Seq: 317 a.a.

Struc: 271 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 211 a.a.*

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 211 a.a.

Protein chain

P62871  (GBB1_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Bos taurus

Seq: 340 a.a.

Struc: 336 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 53 a.a.

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 53 a.a.*

Protein chain

No UniProt id for this chain

Struc: 232 a.a.

* PDB and UniProt seqs differ at 404 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, G: E.C.3.6.5.- - ?????