PDBsum entry 8xzf

Membrane protein

PDB id: 8xzf

Contents

Protein chains

303 a.a.

217 a.a.

339 a.a.

58 a.a.

231 a.a.

14 a.a.

PDB id:

8xzf

Name:

Membrane protein

Title:

Cryo-em structure of the wn561-bound human aplnr-gi complex

Structure:

Apelin receptor. Chain: r. Synonym: angiotensin receptor-like 1,g-protein coupled receptor apj, g-protein coupled receptor hg11. Engineered: yes. Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: aplnr, agtrl1, apj. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell: sf9. Gene: gnai1. Gene: gnb1.

Authors:

W.Wang,S.Ji,Y.Zhang

Key ref:

W.W.Wang et al. (2024). Structure-Based design of non-Hypertrophic apelin rec modulator.. Cell, 187, 1460. PubMed id: 38428423

Date:

21-Jan-24 Release date: 20-Mar-24

Protein chain

P35414  (APJ_HUMAN) -  Apelin receptor from Homo sapiens

Seq: 380 a.a.

Struc: 303 a.a.

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 217 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 339 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 58 a.a.

Protein chain

No UniProt id for this chain

Struc: 231 a.a.

Protein chain

No UniProt id for this chain

Struc: 14 a.a.

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????