PDBsum entry 8ugw

Membrane protein

PDB id: 8ugw

Contents

Protein chain

385 a.a.

Ligands

NGI

PDB id:

8ugw

Name:

Membrane protein

Title:

Computational design of highly signaling active membrane receptors through de novo solvent-mediated allosteric networks

Structure:

Endolysin,adenosine receptor a2a. Chain: a. Fragment: residues 2-322. Engineered: yes

Source:

Tequatrovirus t4, homo sapiens. Human. Organism_taxid: 10665, 9606. Gene: adora2a, adora2. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108

Resolution:

3.90Å R-factor: 0.300 R-free: 0.330

Authors:

J.Wang,K.Y.Chen,J.K.Lai,A.M.Russell,K.Conners,M.E.Rutter,B.Condon, F.Tung,L.Kodandapani,B.Chau,X.Zhao,J.Benach,K.Baker,E.J.Hembre, P.Barth

Key ref:

K.Y.Chen et al. Computational design of highly signaling active membr receptors through solvent-Mediated allosteric network. To be published, . PubMed id: 39849110

Date:

06-Oct-23 Release date: 18-Dec-24

Protein chain

P00720  (ENLYS_BPT4) -  Endolysin from Enterobacteria phage T4

Seq: 164 a.a.

Struc: 385 a.a.*

Protein chain

P29274  (AA2AR_HUMAN) -  Adenosine receptor A2a from Homo sapiens

Seq: 412 a.a.

Struc: 385 a.a.*

* PDB and UniProt seqs differ at 13 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.17 - lysozyme.
• Reaction: Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.