PDBsum entry 8tb0

Signaling protein

PDB id: 8tb0

Contents

Protein chains

233 a.a.

474 a.a.

333 a.a.

50 a.a.

PDB id:

8tb0

Name:

Signaling protein

Title:

Cryo-em structure of gpr61-g protein complex stabilized by scfv16

Structure:

Single-chain fv16. Chain: f. Engineered: yes. Gpr61 fused to dominant negative g alpha s/i n18 chimera. Chain: r. Synonym: biogenic amine receptor-like g-protein coupled receptor, adenylate cyclase-stimulating g alpha protein. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562. Escherichia coli, homo sapiens. Organism_taxid: 562, 9606. Gene: gpr61, balgr, gpcr3, gnas, gnas1, gsp. Expressed in: spodoptera frugiperda.

Authors:

J.A.Lees,J.M.Dias,S.Han

Key ref:

J.A.Lees et al. (2023). An inverse agonist of orphan receptor gpr61 acts by a protein-Competitive allosteric mechanism.. Nat commun, 14, 5938. PubMed id: 37741852

Date:

28-Jun-23 Release date: 04-Oct-23

Protein chain

No UniProt id for this chain

Struc: 233 a.a.

Protein chain

P0ABE7  (C562_ECOLX) -  Soluble cytochrome b562 from Escherichia coli

Seq: 128 a.a.

Struc: 474 a.a.*

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 474 a.a.*

Protein chain

Q9BZJ8  (GPR61_HUMAN) -  G-protein coupled receptor 61 from Homo sapiens

Seq: 451 a.a.

Struc: 474 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 333 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 50 a.a.

* PDB and UniProt seqs differ at 400 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain R: E.C.3.6.5.- - ?????