PDBsum entry 8s2t

Membrane protein

PDB id: 8s2t

Contents

Protein chains

196 a.a.

338 a.a.

57 a.a.

126 a.a.

285 a.a.

Ligands

5FW

PDB id:

8s2t

Name:

Membrane protein

Title:

Cryo-em structure of cell-free synthesized human beta-1 adrenergic receptor cotranslationally inserted into a lipidic nanodiscs

Structure:

Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short. Chain: a. Synonym: adenylate cyclase-stimulating g alpha protein. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnas, gnas1, gsp. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Gene: gnb1. Gene: gng2. Lama glama.

Authors:

F.Merino,Z.Koeck,U.Ermel,P.Dahlhaus,V.Doetsch,J.Kubicek, A.S.Frangakis,D.Hilger,F.Bernhard

Key ref:

F.Merino et al. Cryo-Em structure of cell-Free synthesized human beta adrenergic receptor cotranslationally inserted into a lipidic nanodiscs. To be published, . PubMed id: 40858117

Date:

19-Feb-24 Release date: 21-May-25

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 196 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 338 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 57 a.a.

Protein chain

No UniProt id for this chain

Struc: 126 a.a.

Protein chain

P08588  (ADRB1_HUMAN) -  Beta-1 adrenergic receptor from Homo sapiens

Seq: 477 a.a.

Struc: 285 a.a.*

* PDB and UniProt seqs differ at 38 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????