PDBsum entry 8pok

Membrane protein

PDB id: 8pok

Contents

Protein chains

278 a.a.

219 a.a.

339 a.a.

60 a.a.

128 a.a.

Ligands

HSM

PDB id:

8pok

Name:

Membrane protein

Title:

Cryo-em structure of cell-free synthesized human histamine h2 receptor coupled to heterotrimeric gs protein in lipid environment

Structure:

Histamine h2 receptor. Chain: a. Synonym: h2r,hh2r,gastric receptor i. Engineered: yes. Isoform gnas-2 of guanine nucleotide-binding protein g(s) subunit alpha isoforms short. Chain: b. Synonym: adenylate cyclase-stimulating g alpha protein. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: hrh2. Expressed in: escherichia coli. Expression_system_taxid: 562. Other_details: cell-free synthesis. Gene: gnas, gnas1, gsp. Expressed in: trichoplusia ni.

Authors:

K.Schnelle,Z.Koeck,M.Persechino,S.Umbach,H.Schihada,D.Januliene, K.Parey,S.Pockes,P.Kolb,V.Doetsch,A.Moeller,D.Hilger,F.Bernhard

Key ref:

Z.Kock et al. (2024). Cryo-Em structure of cell-Free synthesized human hist receptor/g s complex in nanodisc environment.. Nat commun, 15, 1831. PubMed id: 38418462

Date:

05-Jul-23 Release date: 06-Mar-24

Protein chain

P25021  (HRH2_HUMAN) -  Histamine H2 receptor from Homo sapiens

Seq: 359 a.a.

Struc: 278 a.a.

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 219 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 339 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 60 a.a.

Protein chain

No UniProt id for this chain

Struc: 128 a.a.

* PDB and UniProt seqs differ at 38 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain B: E.C.3.6.5.- - ?????