PDBsum entry 8p12

Signaling protein

PDB id: 8p12

Contents

Protein chains

317 a.a.

210 a.a.

316 a.a.

36 a.a.

219 a.a.

218 a.a.

PDB id:

8p12

Name:

Signaling protein

Title:

Cryo-em structure of rhodopsin-gi bound to antibody fragment fab13

Structure:

Rhodopsin. Chain: r. Engineered: yes. Mutation: yes. Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit

Source:

Bos taurus. Cattle. Organism_taxid: 9913. Cell: rod photoreceptor cell. Gene: rho. Expressed in: homo sapiens. Expression_system_taxid: 9606. Expression_system_cell_line: hek293. Expression_system_organ: kidney.

Authors:

F.Pamula,O.Tejero,J.Muehle,R.Thoma,G.F.X.Schertler,J.Marino,C.-J.Tsai

Key ref:

F.Pamula et al. Characterization of two novel antibody fragments for obtaining cryo-Em structures of gpcr-G protein comple. To be published, . PubMed id: 41029899

Date:

11-May-23 Release date: 20-Nov-24

Protein chain

P02699  (OPSD_BOVIN) -  Rhodopsin from Bos taurus

Seq: 348 a.a.

Struc: 317 a.a.*

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 210 a.a.

Protein chain

P62871  (GBB1_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Bos taurus

Seq: 340 a.a.

Struc: 316 a.a.

Protein chain

P02698  (GBG1_BOVIN) -  Guanine nucleotide-binding protein G(T) subunit gamma-T1 from Bos taurus

Seq: 74 a.a.

Struc: 36 a.a.

Protein chain

No UniProt id for this chain

Struc: 219 a.a.

Protein chain

No UniProt id for this chain

Struc: 218 a.a.

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????