PDBsum entry 8k9l

Signaling protein

PDB id: 8k9l

Contents

Protein chains

282 a.a.

224 a.a.

339 a.a.

59 a.a.

235 a.a.

Ligands

TYR-DAL-GLY-MEA-ETA

VV9

PDB id:

8k9l

Name:

Signaling protein

Title:

Full agonist- and positive allosteric modulator-bound mu-type opioid receptor-g protein complex

Structure:

Soluble cytochrome b562,mu-type opioid receptor,mu-type opioid receptor. Chain: r. Synonym: m-or-1,mor-1,mu opiate receptor,mu opioid receptor,mop,hmop. Engineered: yes. Mutation: yes. Other_details: n-terminal residues (m1-g63) of mor were replaced with the thermostabilized apocytochrome b562ril from escherichia coli (m7w, h102i and r106l) (bril) protein and a linker sequence

Source:

Escherichia coli, homo sapiens. Human. Organism_taxid: 562, 9606. Gene: cybc, oprm1, mor1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.

Authors:

T.Hisano,T.Uchikubo-Kamo,M.Shirouzu,S.Imai,S.Kaneko,I.Shimada

Key ref:

S.Kaneko et al. (2024). Structural and dynamic insights into the activation o mu-Opioid receptor by an allosteric modulator.. Nat commun, 15, 3544. PubMed id: 38740791

Date:

01-Aug-23 Release date: 29-May-24

Protein chain

P0ABE7  (C562_ECOLX) -  Soluble cytochrome b562 from Escherichia coli

Seq: 128 a.a.

Struc: 282 a.a.*

Protein chain

P35372  (OPRM_HUMAN) -  Mu-type opioid receptor from Homo sapiens

Seq: 400 a.a.

Struc: 282 a.a.*

Protein chain

P08754  (GNAI3_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-3 from Homo sapiens

Seq: 354 a.a.

Struc: 224 a.a.

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 339 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 59 a.a.

Protein chain

No UniProt id for this chain

Struc: 235 a.a.

* PDB and UniProt seqs differ at 116 residue positions (black crosses)