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PDBsum entry 8k4s
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protein ligands Protein-protein interface(s) links
Membrane protein PDB id
8k4s

 

 

 

 

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Contents
Protein chains
255 a.a.
222 a.a.
338 a.a.
47 a.a.
231 a.a.
Ligands
JW0
PDB id:
8k4s
Name: Membrane protein
Title: Cryoem structure of gq coupled mrgprx4 with agonist dca-3p
Structure: Soluble cytochrome b562,mas-related g-protein coupled receptor member x4,green fluorescent protein. Chain: e. Synonym: cytochrome b-562,sensory neuron-specific g-protein coupled receptor 5/6. Engineered: yes. Other_details: the domain (1-8) is corresponding to flag-tag. The doamin (9-116) is corresponding to soluble cytochrome b562. The domain (117-438) is corresponding to mrgprx4 (genbank: ay042216.1).
Source: Escherichia coli, homo sapiens, aequorea victoria. Human, water jellyfish, mesonema victoria. Organism_taxid: 562, 9606, 6100. Gene: cybc, mrgprx4, mrgx4, snsr5, snsr6, gfp. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Homo sapiens. Human.
Authors: J.Yang,J.P.Fan,X.G.Lei
Key ref: J.Yang et al. Structure-Guided discovery of bile acid derivatives f treating liver diseases without causing itch. Cell(cambridge,Mass ), . PubMed id: 39476841
Date:
20-Jul-23     Release date:   06-Nov-24    
PROCHECK
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 Headers
 References

Protein chain
P0ABE7  (C562_ECOLX) -  Soluble cytochrome b562 from Escherichia coli
Seq:
Struc: 		128 a.a.
255 a.a.*
Protein chain
P42212  (GFP_AEQVI) -  Green fluorescent protein from Aequorea victoria
Seq:
Struc: 		238 a.a.
255 a.a.*
Protein chain
Q96LA9  (MRGX4_HUMAN) -  Mas-related G-protein coupled receptor member X4 from Homo sapiens
Seq:
Struc: 		322 a.a.
255 a.a.
Protein chain
A0A590UJY2  (A0A590UJY2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha from Homo sapiens
Seq:
Struc: 		237 a.a.
222 a.a.*
Protein chain
P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens
Seq:
Struc: 		340 a.a.
338 a.a.
Protein chain
P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens
Seq:
Struc: 		71 a.a.
47 a.a.
Protein chain
No UniProt id for this chain
Struc: 231 a.a.
Key:    Secondary structure
* PDB and UniProt seqs differ at 263 residue positions (black crosses)

 

 

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