PDBsum entry 8i7v

Membrane protein

PDB id: 8i7v

Contents

Protein chains

291 a.a.

145 a.a.

339 a.a.

58 a.a.

233 a.a.

Ligands

OJX

PDB id:

8i7v

Name:

Membrane protein

Title:

Cryo-em structure of acipimox bound human hydroxy-carboxylic acid receptor 2 in complex with gi heterotrimer

Structure:

Human hydroxycarboxylic acid receptor 2,hydroxycarboxylic acid receptor 2,hydroxycarboxylic acid receptor 2. Chain: a. Synonym: g-protein coupled receptor 109a,g-protein coupled receptor hm74a,niacin receptor 1,nicotinic acid receptor. Engineered: yes. Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: b. Synonym: adenylate cyclase-inhibiting g alpha protein.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: hcar2, gpr109a, hca2, hm74a, niacr1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gnai1. Gene: gnb1. Gene: gng2.

Authors:

J.H.Park,N.Ishimoto,S.Y.Park

Key ref:

J.H.Park et al. (2023). Structural basis for ligand recognition and signaling hydroxy-Carboxylic acid receptor 2.. Nat commun, 14, 7150. PubMed id: 37932263

Date:

02-Feb-23 Release date: 07-Feb-24

Protein chain

P0ABE7  (C562_ECOLX) -  Soluble cytochrome b562 from Escherichia coli

Seq: 128 a.a.

Struc: 291 a.a.*

Protein chain

Q8TDS4  (HCAR2_HUMAN) -  Hydroxycarboxylic acid receptor 2 from Homo sapiens

Seq: 363 a.a.

Struc: 291 a.a.

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 145 a.a.

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 339 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 58 a.a.

Protein chain

No UniProt id for this chain

Struc: 233 a.a.

* PDB and UniProt seqs differ at 112 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain B: E.C.3.6.5.- - ?????