PDBsum entry 8gfy

Signaling protein

PDB id: 8gfy

Contents

Protein chains

230 a.a.

331 a.a.

52 a.a.

278 a.a.

Ligands

GTP

G1I

Waters ×4

PDB id:

8gfy

Name:

Signaling protein

Title:

Cryoem structure of beta-2-adrenergic receptor in complex with gtp- bound gs heterotrimer (transition intermediate #4 of 20)

Structure:

Guanine nucleotide-binding protein g(s) subunit alpha isoforms short. Chain: a. Synonym: adenylate cyclase-stimulating g alpha protein. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnas, gnas1, gsp. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Gene: gnb1. Gene: gng2. Gene: adrb2, adrb2r, b2ar.

Authors:

M.M.Papasergi-Scott,G.Skiniotis

Key ref:

M.M.Papasergi-Scott et al. Time-Resolved cryo-Em of g protein activation by a gp. Nature, . PubMed id: 38480881

Date:

08-Mar-23 Release date: 06-Mar-24

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 230 a.a.

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 331 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 52 a.a.

Protein chain

P07550  (ADRB2_HUMAN) -  Beta-2 adrenergic receptor from Homo sapiens

Seq: 413 a.a.

Struc: 278 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????