PDBsum entry 8gcp

Membrane protein

PDB id: 8gcp

Contents

Protein chains

216 a.a.

337 a.a.

55 a.a.

229 a.a.

271 a.a.

Ligands

P2E

PDB id:

8gcp

Name:

Membrane protein

Title:

Cryo-em structure of the prostaglandin e2 receptor 4 coupled to g protein

Structure:

Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnai1. Expressed in: baculovirus expression vector pfastbac1-hm. Expression_system_taxid: 274590. Gene: gnb1. Gene: mrhifer1_005810. Gene: ptger4, ptger2.

Authors:

S.M.Huang,M.Y.Xiong,L.Liu,J.Mu,C.Sheng,J.Sun

Key ref:

S.M.Huang et al. (2023). Single hormone or synthetic agonist induces gs/gi cou selectivity of ep receptors via distinct binding mode propagating paths. Proc natl acad sci usa, 120, . PubMed id: 37478163

Date:

02-Mar-23 Release date: 03-Jan-24

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 216 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 337 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 55 a.a.

Protein chain

No UniProt id for this chain

Struc: 229 a.a.

Protein chain

P35408  (PE2R4_HUMAN) -  Prostaglandin E2 receptor EP4 subtype from Homo sapiens

Seq: 488 a.a.

Struc: 271 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????