PDBsum entry 8fx5

Membrane protein/signaling protein

PDB id: 8fx5

Contents

Protein chains

271 a.a.

339 a.a.

57 a.a.

235 a.a.

225 a.a.

Ligands

XNO ×2

Waters ×47

PDB id:

8fx5

Name:

Membrane protein/signaling protein

Title:

Human m4 muscarinic acetylcholine receptor complex with gi1 and xanomeline

Structure:

Muscarinic acetylcholine receptor m4. Chain: r. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: chrm4. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gnb1. Gene: gng2. Expressed in: trichoplusia ni.

Authors:

Z.Vuckovic,J.I.Mobbs,A.Glukhova,P.M.Sexton,R.Danev,D.M.Thal

Key ref:

W.A.C.Burger et al. (2023). Xanomeline displays concomitant orthosteric and allos binding modes at the m 4 machr.. Nat commun, 14, 5440. PubMed id: 37673901

Date:

23-Jan-23 Release date: 13-Sep-23

Protein chain

P08173  (ACM4_HUMAN) -  Muscarinic acetylcholine receptor M4 from Homo sapiens

Seq: 479 a.a.

Struc: 271 a.a.

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 339 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 57 a.a.

Protein chain

No UniProt id for this chain

Struc: 235 a.a.

Protein chain

B2RSH2  (GNAI1_MOUSE) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Mus musculus

Seq: 354 a.a.

Struc: 225 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????