PDBsum entry 8eit

Membrane protein

PDB id: 8eit

Contents

Protein chains

226 a.a.

336 a.a.

59 a.a.

234 a.a.

268 a.a.

Ligands

HXA

PDB id:

8eit

Name:

Membrane protein

Title:

Structure of ffar1-gq complex bound to dha

Structure:

A modified guanine nucleotide-binding protein g(q) subunit alpha. Chain: a. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9. Gene: gnb1. Gene: gng2. Tequatrovirus t4, homo sapiens.

Authors:

P.Kumari,A.Inoue,K.Chapman,P.Lian,D.M.Rosenbaum

Key ref:

P.Kumari et al. (2023). Molecular mechanism of fatty acid activation of ffar1. Proc natl acad sci usa, 120, 69120. PubMed id: 37216523

Date:

15-Sep-22 Release date: 24-May-23

Protein chain

No UniProt id for this chain

Struc: 226 a.a.

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 336 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 59 a.a.

Protein chain

No UniProt id for this chain

Struc: 234 a.a.

Protein chain

O14842  (FFAR1_HUMAN) -  Free fatty acid receptor 1 from Homo sapiens

Seq: 300 a.a.

Struc: 268 a.a.

Protein chain

P00720  (ENLYS_BPT4) -  Endolysin from Enterobacteria phage T4

Seq: 164 a.a.

Struc: 268 a.a.*

* PDB and UniProt seqs differ at 143 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain R: E.C.3.2.1.17 - lysozyme.
• Reaction: Hydrolysis of the 1,4-beta-linkages between N-acetyl-D-glucosamine and N-acetylmuramic acid in peptidoglycan heteropolymers of the prokaryotes cell walls.