PDBsum entry 8ef5

Signaling protein

PDB id: 8ef5

Contents

Protein chains

287 a.a.

225 a.a.

336 a.a.

56 a.a.

231 a.a.

18 a.a.

Ligands

7V7 ×2

CLR ×10

PDB id:

8ef5

Name:

Signaling protein

Title:

Fentanyl-bound mu-opioid receptor-gi complex

Structure:

Mu-type opioid receptor. Chain: r, m. Synonym: m-or-1,mor-1,mu opiate receptor,mu opioid receptor,mop,hmop. Engineered: yes. Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a, f. Synonym: adenylate cyclase-inhibiting g alpha protein. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: oprm1, mor1. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gnai1. Rattus norvegicus. Rat.

Authors:

Y.Zhuang,Y.Wang,S.Guo,X.E.Zhou,Q.Rao,X.He,B.He,J.Liu,Q.Zhou,X.Wang, W.Liu,X.Jiang,D.Yang,X.Chen,Y.Jiang,H.Jiang,J.Shen,K.Melcher,M.Wang, X.Xie,H.E.Xu

Key ref:

Y.Zhuang et al. Molecular recognition of morphine and fentanyl by the mu-Opioid receptor. Cell(cambridge,Mass ), . PubMed id: 36368306

Date:

08-Sep-22 Release date: 09-Nov-22

Protein chains

P35372  (OPRM_HUMAN) -  Mu-type opioid receptor from Homo sapiens

Seq: 400 a.a.

Struc: 287 a.a.

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 225 a.a.*

Protein chain

P54311  (GBB1_RAT) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Rattus norvegicus

Seq: 340 a.a.

Struc: 336 a.a.

Protein chain

P63212  (GBG2_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Bos taurus

Seq: 71 a.a.

Struc: 56 a.a.

Protein chain

No UniProt id for this chain

Struc: 231 a.a.

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 18 a.a.

* PDB and UniProt seqs differ at 2 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chains A, F: E.C.3.6.5.- - ?????