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UniProt functional annotation for Q8Y8D7 | ||
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| UniProt code: Q8Y8D7. |
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| Organism: | |
Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e). |
| Taxonomy: | |
Bacteria; Firmicutes; Bacilli; Bacillales; Listeriaceae; Listeria. |
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| Function: | |
Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP. {ECO:0000255|HAMAP- Rule:MF_00361, ECO:0000269|PubMed:17686780, ECO:0000269|PubMed:22608967}. |
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| Catalytic activity: | |
Reaction=ATP + NAD(+) = ADP + H(+) + NADP(+); Xref=Rhea:RHEA:18629, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57540, ChEBI:CHEBI:58349, ChEBI:CHEBI:456216; EC=2.7.1.23; Evidence={ECO:0000255|HAMAP-Rule:MF_00361, ECO:0000269|PubMed:17686780}; |
| Cofactor: | |
Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Evidence={ECO:0000255|HAMAP-Rule:MF_00361}; |
| Activity regulation: | |
Competitively inhibited by 5'-thioacetyladenosine (TAA) and di-(5'-thioadenosine) (DTA). {ECO:0000269|PubMed:17686780, ECO:0000269|PubMed:22608967}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=2.8 mM for ATP (at pH 7.5 and 30 degrees Celsius) {ECO:0000269|PubMed:17686780}; Vmax=6.67 umol/min/mg enzyme (at pH 7.5 and 30 degrees Celsius) {ECO:0000269|PubMed:17686780}; Note=Kcat is 13.78 sec(-1) for kinase activity with ATP (at pH 7.5 and 30 degrees Celsius). Kcat is 13.12 sec(-1) for kinase activity with NAD (at pH 7.5 and 30 degrees Celsius).; |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:17686780, ECO:0000269|PubMed:22608967}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00361}. |
| Similarity: | |
Belongs to the NAD kinase family. {ECO:0000255|HAMAP- Rule:MF_00361}. |
Annotations taken from UniProtKB at the EBI.