PDBsum entry 7zpa

DNA binding protein

PDB id: 7zpa

Contents

Protein chains

431 a.a.

DNA/RNA

PDB id:

7zpa

Name:

DNA binding protein

Title:

Cryo-em structure of holo-pdxr from bacillus clausii bound to its target DNA in the closed conformation, c1 symmetry

Structure:

Plp-dependent aminotransferase family protein. Chain: a, b. Engineered: yes. DNA (48-mer). Chain: c. Engineered: yes. DNA (48-mer). Chain: d. Engineered: yes

Source:

Alkalihalobacillus clausii. Organism_taxid: 79880. Gene: chh72_17460. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Synthetic: yes. Organism_taxid: 79880

Authors:

I.Freda,L.C.Montemiglio,A.Tramonti,R.Contestabile,B.Vallone, C.Exertier,C.Savino,A.Chaves Sanjuan,M.Bolognesi

Key ref:

I.Freda et al. Cryo-Em structure of holo-Pdxr from bacillus clausii to its target DNA in the closed conformation, C2 symm. To be published, . PubMed id: 37378428

Date:

27-Apr-22 Release date: 05-Jul-23

Protein chains

Q5WKW3  (Q5WKW3_BACSK) -  GntR family transcriptional regulator from Alkalihalobacillus clausii (strain KSM-K16)

Seq: 464 a.a.

Struc: 431 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

DNA/RNA chains

C-T-G-A-C-C-T-C-A-T-C-A-T-T-T-T-C-T-T-A-A-A-A-A-C-T-G-A-C-A-C-T-T-A-C-A-A-T-G- 48 bases

A-C-T-G-A-C-C-A-C-A-T-T-G-T-A-A-G-T-G-T-C-A-G-T-T-T-T-T-A-A-G-A-A-A-A-T-G-A-T- 47 bases

Enzyme reactions

• Enzyme class: E.C.?