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UniProt functional annotation for P07329 | ||
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| UniProt code: P07329. |
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| Organism: | |
Azotobacter vinelandii. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Azotobacter. |
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| Function: | |
This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation. |
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| Catalytic activity: | |
Reaction=16 ATP + 16 H2O + N2 + 8 reduced [2Fe-2S]-[ferredoxin] = 16 ADP + 6 H(+) + H2 + 2 NH4(+) + 8 oxidized [2Fe-2S]-[ferredoxin] + 16 phosphate; Xref=Rhea:RHEA:21448, Rhea:RHEA-COMP:10000, Rhea:RHEA- COMP:10001, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:17997, ChEBI:CHEBI:18276, ChEBI:CHEBI:28938, ChEBI:CHEBI:30616, ChEBI:CHEBI:33737, ChEBI:CHEBI:33738, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=1.18.6.1; |
| Cofactor: | |
Name=[8Fe-7S] cluster; Xref=ChEBI:CHEBI:21143; Note=Binds 1 [8Fe-7S] cluster per heterodimer.; |
| Activity regulation: | |
Nitrogenase holoenzyme is subject to 'conformational protection' by FeSII; under oxidizing conditions FeSII binds to the holoenzyme and reversibly protects it from oxidation (PubMed:7830548). {ECO:0000269|PubMed:7830548}. |
| Subunit: | |
Tetramer of two alpha and two beta chains. Forms complex with the iron protein (nitrogenase component 2). |
| Induction: | |
Constitutively expressed during log and stationary phase in sucrose-limited cultures, its levels decrease during stationary phase (at protein level). {ECO:0000269|PubMed:7830548}. |
| Similarity: | |
Belongs to the NifD/NifK/NifE/NifN family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.