UniProt functional annotation for Q6HA08



	UniProt functional annotation for Q6HA08

UniProt code: Q6HA08.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding (By similarity). {ECO:0000250}.

Cofactor: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000255|PROSITE-ProRule:PRU01211}; Note=Binds 1 zinc ion per subunit. {ECO:0000255|PROSITE- ProRule:PRU01211};

Activity regulation: Inhibited by wide spectrum metalloproteinase inhibitor batimastat (BB-94). Also inhibited by EDTA. {ECO:0000269|PubMed:15087446}.

Biophysicochemical properties: Kinetic parameters: Note=Enzymatic activity was assayed using synthetic quenched fluorescent substrates QF-24, QF-35 and QF-41. Astacin displayed the highest Kcat/KM value against QF-35. {ECO:0000269|PubMed:15087446}; pH dependence: Optimum pH is 7.5. {ECO:0000269|PubMed:15087446};

Subunit: Interacts (via N-terminal domain) with SPACA3; the interaction occurs during fertilization. {ECO:0000250}.

Subcellular location: Cytoplasm {ECO:0000250|UniProtKB:Q6HA09}. Cell membrane {ECO:0000250|UniProtKB:Q6HA09}. Cytoplasmic vesicle, secretory vesicle, Cortical granule {ECO:0000250|UniProtKB:Q6HA09}. Note=Probably exocytosed from cortical granules during post-fertilization. Detected throughout the ooplasm of germinal vesicle stage oocytes in early bilaminar secondary follicles at postnatal (PN) day 3. Detected in the microvillar domain of the oolemma in arrested ovulated secondary oocytes and in the first polar body prior to fertilization. Upon fertilization, detected in the perivitelline space (PVS) and occasionally on the oolemma in 2-cell through morulae stages. Colocalizes with SPACA3 at the microvillar domain of the oolemma and in the perivitelline space (PVS). {ECO:0000250|UniProtKB:Q6HA09}.

Tissue specificity: Expressed in promyelocytic leukemia HL-60 cells, Burkitt's lymphoma Raji cells, and also in some ovarian carcinomas. Not detected in normal tissues. {ECO:0000269|PubMed:15087446}.

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding (By similarity). {ECO:0000250}.


Cofactor:		Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000255\|PROSITE-ProRule:PRU01211}; Note=Binds 1 zinc ion per subunit. {ECO:0000255\|PROSITE- ProRule:PRU01211};
Activity regulation:		Inhibited by wide spectrum metalloproteinase inhibitor batimastat (BB-94). Also inhibited by EDTA. {ECO:0000269\|PubMed:15087446}.
Biophysicochemical properties:		Kinetic parameters: Note=Enzymatic activity was assayed using synthetic quenched fluorescent substrates QF-24, QF-35 and QF-41. Astacin displayed the highest Kcat/KM value against QF-35. {ECO:0000269\|PubMed:15087446}; pH dependence: Optimum pH is 7.5. {ECO:0000269\|PubMed:15087446};
Subunit:		Interacts (via N-terminal domain) with SPACA3; the interaction occurs during fertilization. {ECO:0000250}.
Subcellular location:		Cytoplasm {ECO:0000250\|UniProtKB:Q6HA09}. Cell membrane {ECO:0000250\|UniProtKB:Q6HA09}. Cytoplasmic vesicle, secretory vesicle, Cortical granule {ECO:0000250\|UniProtKB:Q6HA09}. Note=Probably exocytosed from cortical granules during post-fertilization. Detected throughout the ooplasm of germinal vesicle stage oocytes in early bilaminar secondary follicles at postnatal (PN) day 3. Detected in the microvillar domain of the oolemma in arrested ovulated secondary oocytes and in the first polar body prior to fertilization. Upon fertilization, detected in the perivitelline space (PVS) and occasionally on the oolemma in 2-cell through morulae stages. Colocalizes with SPACA3 at the microvillar domain of the oolemma and in the perivitelline space (PVS). {ECO:0000250\|UniProtKB:Q6HA09}.
Tissue specificity:		Expressed in promyelocytic leukemia HL-60 cells, Burkitt's lymphoma Raji cells, and also in some ovarian carcinomas. Not detected in normal tissues. {ECO:0000269\|PubMed:15087446}.