UniProt functional annotation for P33599



	UniProt functional annotation for P33599

UniProt code: P33599.

Organism: Escherichia coli (strain K12).

Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.

Function: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.

Catalytic activity: Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; Evidence={ECO:0000255|HAMAP-Rule:MF_01359};

Subunit: NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.

Subcellular location: Cytoplasm {ECO:0000269|PubMed:16079137}. Cell inner membrane {ECO:0000255|HAMAP-Rule:MF_01359, ECO:0000269|PubMed:16079137}; Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01359, ECO:0000269|PubMed:16079137}.

Similarity: In the N-terminal section; belongs to the complex I 30 kDa subunit family. {ECO:0000255|HAMAP-Rule:MF_01359}.

Similarity: In the C-terminal section; belongs to the complex I 49 kDa subunit family. {ECO:0000255|HAMAP-Rule:MF_01359}.

Sequence caution: Sequence=AAA03535.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAA16115.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=CAA48363.1; Type=Frameshift; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Escherichia coli (strain K12).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.



Function:		NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.


Catalytic activity:		Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124; Evidence={ECO:0000255\|HAMAP-Rule:MF_01359};
Subunit:		NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.
Subcellular location:		Cytoplasm {ECO:0000269\|PubMed:16079137}. Cell inner membrane {ECO:0000255\|HAMAP-Rule:MF_01359, ECO:0000269\|PubMed:16079137}; Peripheral membrane protein {ECO:0000255\|HAMAP-Rule:MF_01359, ECO:0000269\|PubMed:16079137}.
Similarity:		In the N-terminal section; belongs to the complex I 30 kDa subunit family. {ECO:0000255\|HAMAP-Rule:MF_01359}.
Similarity:		In the C-terminal section; belongs to the complex I 49 kDa subunit family. {ECO:0000255\|HAMAP-Rule:MF_01359}.
Sequence caution:		Sequence=AAA03535.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAA16115.2; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; Sequence=CAA48363.1; Type=Frameshift; Evidence={ECO:0000305};