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PDBsum entry 7nol
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Viral protein
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PDB id
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7nol
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Contents |
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78 a.a.
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217 a.a.
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139 a.a.
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References listed in PDB file
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Key reference
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Title
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Structure of the mature rous sarcoma virus lattice reveals a role for ip6 in the formation of the capsid hexamer.
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Authors
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M.Obr,
C.L.Ricana,
N.Nikulin,
J.R.Feathers,
M.Klanschnig,
A.Thader,
M.C.Johnson,
V.M.Vogt,
F.K.M.Schur,
R.A.Dick.
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Ref.
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Nat Commun, 2021,
12,
3226.
[DOI no: ]
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PubMed id
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Abstract
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Inositol hexakisphosphate (IP6) is an assembly cofactor for HIV-1. We report
here that IP6 is also used for assembly of Rous sarcoma virus (RSV), a
retrovirus from a different genus. IP6 is ~100-fold more potent at promoting RSV
mature capsid protein (CA) assembly than observed for HIV-1 and removal of IP6
in cells reduces infectivity by 100-fold. Here, visualized by cryo-electron
tomography and subtomogram averaging, mature capsid-like particles show an
IP6-like density in the CA hexamer, coordinated by rings of six lysines and six
arginines. Phosphate and IP6 have opposing effects on CA in vitro assembly,
inducing formation of Tâ=â1 icosahedrons and tubes, respectively, implying
that phosphate promotes pentamer and IP6 hexamer formation. Subtomogram
averaging and classification optimized for analysis of pleomorphic retrovirus
particles reveal that the heterogeneity of mature RSV CA polyhedrons results
from an unexpected, intrinsic CA hexamer flexibility. In contrast, the CA
pentamer forms rigid units organizing the local architecture. These different
features of hexamers and pentamers determine the structural mechanism to form CA
polyhedrons of variable shape in mature RSV particles.
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