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PDBsum entry 7l2c
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Immune system/viral protein
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PDB id
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7l2c
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Contents |
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286 a.a.
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221 a.a.
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213 a.a.
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References listed in PDB file
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Key reference
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Title
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Potent sars-Cov-2 neutralizing antibodies directed against spike n-Terminal domain target a single supersite.
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Authors
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G.Cerutti,
Y.Guo,
T.Zhou,
J.Gorman,
M.Lee,
M.Rapp,
E.R.Reddem,
J.Yu,
F.Bahna,
J.Bimela,
Y.Huang,
P.S.Katsamba,
L.Liu,
M.S.Nair,
R.Rawi,
A.S.Olia,
P.Wang,
B.Zhang,
G.Y.Chuang,
D.D.Ho,
Z.Sheng,
P.D.Kwong,
L.Shapiro.
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Ref.
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Cell Host Microbe, 2021,
29,
819.
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PubMed id
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Abstract
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Numerous antibodies that neutralize SARS-CoV-2 have been identified, and these
generally target either the receptor-binding domain (RBD) or the N-terminal
domain (NTD) of the viral spike. While RBD-directed antibodies have been
extensively studied, far less is known about NTD-directed antibodies. Here, we
report cryo-EM and crystal structures for seven potent NTD-directed neutralizing
antibodies in complex with spike or isolated NTD. These structures defined
several antibody classes, with at least one observed in multiple convalescent
donors. The structures revealed that all seven antibodies target a common
surface, bordered by glycans N17, N74, N122, and N149. This site-formed
primarily by a mobile β-hairpin and several flexible loops-was highly
electropositive, located at the periphery of the spike, and the largest
glycan-free surface of NTD facing away from the viral membrane. Thus, in
contrast to neutralizing RBD-directed antibodies that recognize multiple
non-overlapping epitopes, potent NTD-directed neutralizing antibodies appear to
target a single supersite.
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