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UniProt functional annotation for Q99538 | ||
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| UniProt code: Q99538. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation (By similarity). May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. {ECO:0000250, ECO:0000269|PubMed:23776206}. |
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| Catalytic activity: | |
Reaction=Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.; EC=3.4.22.34; Evidence={ECO:0000269|PubMed:23776206}; |
| Activity regulation: | |
Inhibited by CST6. {ECO:0000269|PubMed:30425301}. |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 5.5 for the free enzyme, and pH 6 in the presence of bound integrins. {ECO:0000269|PubMed:23776206}; |
| Subunit: | |
Homodimer before autocatalytic removal of the propeptide (By similarity). Monomer after autocatalytic processing. May interact with integrins. {ECO:0000250, ECO:0000269|PubMed:23776206}. |
| Subcellular location: | |
Lysosome {ECO:0000250}. |
| Tissue specificity: | |
Ubiquitous. Particularly abundant in kidney, heart and placenta. |
| Domain: | |
In the zymogen form, the uncleaved propeptide blocks access to the active site. {ECO:0000269|PubMed:23776206}. |
| Ptm: | |
Glycosylated. {ECO:0000269|PubMed:19159218, ECO:0000269|PubMed:23776206}. |
| Ptm: | |
Activated by autocatalytic processing at pH 4. {ECO:0000269|PubMed:11085925}. |
| Similarity: | |
Belongs to the peptidase C13 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.