UniProt functional annotation for Q6IVU0



	UniProt functional annotation for Q6IVU0

UniProt code: Q6IVU0.

Organism: Machupo virus (MACV).

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Arenaviridae; Mammarenavirus.

Function: RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner. {ECO:0000256|HAMAP-Rule:MF_04086, ECO:0000256|PIRNR:PIRNR000836}.

Catalytic activity: Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:11128, Rhea:RHEA- COMP:11129, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:83400; EC=2.7.7.48; Evidence={ECO:0000256|HAMAP-Rule:MF_04086, ECO:0000256|PIRNR:PIRNR000836};

Subunit: Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N. Interacts with protein Z; this interaction inhibits viral transcription and replication. {ECO:0000256|HAMAP-Rule:MF_04086, ECO:0000256|PIRNR:PIRNR000836}.

Subcellular location: Virion {ECO:0000256|HAMAP-Rule:MF_04086, ECO:0000256|PIRNR:PIRNR000836}. Host cytoplasm {ECO:0000256|HAMAP- Rule:MF_04086, ECO:0000256|PIRNR:PIRNR000836}.

Domain: The N-terminal domain contains an RNA endonuclease activity that plays an essential role in mRNA transcription. This activity may be involved in cap snatching. {ECO:0000256|HAMAP-Rule:MF_04086}.

Similarity: Belongs to the arenaviridae RNA polymerase family. {ECO:0000256|HAMAP-Rule:MF_04086, ECO:0000256|PIRNR:PIRNR000836}.

Annotations taken from UniProtKB at the EBI.


Organism:		Machupo virus (MACV).
Taxonomy:		Viruses; Riboviria; Orthornavirae; Negarnaviricota; Polyploviricotina; Ellioviricetes; Bunyavirales; Arenaviridae; Mammarenavirus.



Function:		RNA-dependent RNA polymerase which is responsible for replication and transcription of the viral RNA genome. During transcription, synthesizes 4 subgenomic RNAs, and assures their capping by a cap-snatching mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. The 3'-end of subgenomic mRNAs molecules are heterogeneous and not polyadenylated. The replicase function is to direct synthesis of antigenomic and genomic RNA which are encapsidated and non capped. As a consequence of the use of the same enzyme for both transcription and replication, these mechanisms need to be well coordinated. These processes may be regulated by proteins N and Z in a dose-dependent manner. {ECO:0000256\|HAMAP-Rule:MF_04086, ECO:0000256\|PIRNR:PIRNR000836}.


Catalytic activity:		Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:11128, Rhea:RHEA- COMP:11129, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:83400; EC=2.7.7.48; Evidence={ECO:0000256\|HAMAP-Rule:MF_04086, ECO:0000256\|PIRNR:PIRNR000836};
Subunit:		Homomultimerizes; the oligomeric structure is essential for the polymerase activity. Interacts with the nucleocapsid protein N. Interacts with protein Z; this interaction inhibits viral transcription and replication. {ECO:0000256\|HAMAP-Rule:MF_04086, ECO:0000256\|PIRNR:PIRNR000836}.
Subcellular location:		Virion {ECO:0000256\|HAMAP-Rule:MF_04086, ECO:0000256\|PIRNR:PIRNR000836}. Host cytoplasm {ECO:0000256\|HAMAP- Rule:MF_04086, ECO:0000256\|PIRNR:PIRNR000836}.
Domain:		The N-terminal domain contains an RNA endonuclease activity that plays an essential role in mRNA transcription. This activity may be involved in cap snatching. {ECO:0000256\|HAMAP-Rule:MF_04086}.
Similarity:		Belongs to the arenaviridae RNA polymerase family. {ECO:0000256\|HAMAP-Rule:MF_04086, ECO:0000256\|PIRNR:PIRNR000836}.