PDBsum entry 7dsi

Lipid transport

PDB id: 7dsi

Contents

Protein chains

1145 a.a.

362 a.a.

Ligands

NAG-NAG ×2

NAG-NAG-BMA

ACP

Metals

_MG

PDB id:

7dsi

Name:

Lipid transport

Title:

Cryo-em structure of dnf1 from saccharomyces cerevisiae in yeast lipids with amppcp ( resting state )

Structure:

Phospholipid-transporting atpase dnf1. Chain: a. Synonym: flippase dnf1. Engineered: yes. Alkylphosphocholine resistance protein lem3. Chain: b. Synonym: brefeldin-a sensitivity protein 3,ro-sensitive 3. Engineered: yes

Source:

Saccharomyces cerevisiae s288c. Organism_taxid: 559292. Strain: atcc 204508 / s288c. Gene: dnf1, yer166w, sygp-orf7. Expressed in: saccharomyces cerevisiae s288c. Expression_system_taxid: 559292. Gene: lem3, bre3, ros3, ynl323w, n0333. Expression_system_taxid: 559292

Authors:

J.Xu,Y.He,X.Wu,L.Li

Key ref:

J.Xu et al. (2022). Conformational changes of a phosphatidylcholine flipp lipid membranes.. Cell rep, 38, . PubMed id: 35294892

Date:

31-Dec-20 Release date: 23-Mar-22

Protein chain

P32660  (ATC5_YEAST) -  Phospholipid-transporting ATPase DNF1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1571 a.a.

Struc: 1145 a.a.

Protein chain

P42838  (LEM3_YEAST) -  Phospholipid-transporting ATPase accessory subunit LEM3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 414 a.a.

Struc: 362 a.a.

Enzyme reactions

• Enzyme class: Chain A: E.C.7.6.2.1 - P-type phospholipid transporter.
• Reaction: ATP + H2O + phospholipidSide 1 = ADP + phosphate + phospholipidSide 2

ATP + H2O + phospholipidSide 1 = ADP (Bound ligand (Het Group name = ACP) matches with 81.25% similarity) + phosphate + phospholipidSide 2

Molecule diagrams generated from .mol files obtained from the KEGG ftp site