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PDBsum entry 7d8h
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Protein binding
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PDB id
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7d8h
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References listed in PDB file
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Key reference
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Title
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Structural insights into the interaction between crtcs and 14-3-3.
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Authors
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H.Chen,
H.Zhang,
P.Chen,
S.Xiang.
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Ref.
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J Mol Biol, 2021,
433,
166874.
[DOI no: ]
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PubMed id
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Abstract
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The CREB-Regulated Transcriptional Coactivators (CRTCs) regulate the
transcription of CREB target genes and have important functions in many
biological processes. At the basal state, they are phosphorylated at multiple
residues, which promotes their association with 14-3-3 that sequesters them in
the cytoplasm. Upon dephosphorylation, they translocate into the nuclei and
associate with CREB to activate the target gene transcription. Although three
conserved serine residues in CRTCs have been implicated in their phosphorylation
regulation, whether and how they mediate interactions with 14-3-3 is unclear.
Here, we provide direct evidence that these residues and flanking regions
interact with 14-3-3 and the structural basis of the interaction. Our study also
identified a novel salt bridge in CRTC1 with an important function in binding
14-3-3, expanding the understanding of the interaction between 14-3-3 and its
ligands.
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