PDBsum entry 7d69

Nuclear protein

PDB id: 7d69

Contents

Protein chains

90 a.a.

78 a.a.

96 a.a.

99 a.a.

95 a.a.

DNA/RNA

PDB id:

7d69

Name:

Nuclear protein

Title:

Cryo-em structure of the nucleosome containing giardia histones

Structure:

Histone h3. Chain: a, e. Engineered: yes. Histone h4. Chain: b, f. Engineered: yes. Histone h2b. Chain: d, h. Engineered: yes.

Source:

Giardia intestinalis. Organism_taxid: 5741. Gene: dha2_154455. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: dha2_135001, dha2_154716, gsb_155602. Gene: dha2_151009. Synthetic construct. Organism_taxid: 32630.

Authors:

S.Sato,Y.Takizawa,H.Kurumizaka

Key ref:

S.Sato et al. (2021). Cryo-Em structure of the nucleosome core particle con giardia lamblia histones.. Nucleic acids res, 49, 8934. PubMed id: 34352093

Date:

29-Sep-20 Release date: 08-Sep-21

Protein chain

E2RU29  (E2RU29_GIAIC) -  Histone H3 from Giardia intestinalis (strain ATCC 50803 / WB clone C6)

Seq: 146 a.a.

Struc: 90 a.a.

Protein chains

A8BUJ9  (A8BUJ9_GIAIC) -  Histone H4 from Giardia intestinalis (strain ATCC 50803 / WB clone C6)

Seq: 99 a.a.

Struc: 78 a.a.

Protein chains

A8BI78  (A8BI78_GIAIC) -  Histone H2B from Giardia intestinalis (strain ATCC 50803 / WB clone C6)

Seq: 130 a.a.

Struc: 96 a.a.

Protein chain

E2RU29  (E2RU29_GIAIC) -  Histone H3 from Giardia intestinalis (strain ATCC 50803 / WB clone C6)

Seq: 146 a.a.

Struc: 99 a.a.

Protein chains

A8BHS7  (A8BHS7_GIAIC) -  Histone H2A from Giardia intestinalis (strain ATCC 50803 / WB clone C6)

Seq: 124 a.a.

Struc: 95 a.a.

DNA/RNA chains

T-C-C-C-G-G-T-G-C-C-G-A-G-G-C-C-G-C-T-C-A-A-T-T-G-G-T-C-G-T-A-G-A-C-A-G-C-T-C- 125 bases

T-G-C-C-G-A-G-G-C-C-G-C-T-C-A-A-T-T-G-G-T-C-G-T-A-G-A-C-A-G-C-T-C-T-A-G-C-A-C- 125 bases