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UniProt functional annotation for B7QK46 | ||
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| UniProt code: B7QK46. |
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| Organism: | |
Ixodes scapularis (Black-legged tick) (Deer tick). |
| Taxonomy: | |
Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari; Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Ixodinae; Ixodes. |
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| Function: | |
Responsible for the biosynthesis of pyroglutamyl peptides (PubMed:23770496, PubMed:24598748). Seems to have a preference for substrates with neutral or hydrophobic amino-acid residues at the second and third positions (PubMed:24598748). Shows activity towards the peptides [Gln-1]-corazonin, [Gln-1]-periviscerokinin and [Gln-1]- sulfakinin (PubMed:24598748). {ECO:0000269|PubMed:23770496, ECO:0000269|PubMed:24598748}. |
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| Catalytic activity: | |
Reaction=N-terminal L-glutaminyl-[peptide] = N-terminal 5-oxo-L-prolyl- [peptide] + NH4(+); Xref=Rhea:RHEA:23652, Rhea:RHEA-COMP:11736, Rhea:RHEA-COMP:11846, ChEBI:CHEBI:28938, ChEBI:CHEBI:64722, ChEBI:CHEBI:87215; EC=2.3.2.5; Evidence={ECO:0000269|PubMed:23770496, ECO:0000269|PubMed:24598748}; |
| Activity regulation: | |
Strongly inhibited by PBD150, 1-benzylimidazole and N-omega-acetylhistamine, in a Zn(2+)-independent manner. {ECO:0000269|PubMed:24598748}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=21.8 uM for L-glutaminyl 2-naphthylamide (Gln-beta-NA) (in the presence of Zn(2+), at 25 degrees Celsius) {ECO:0000269|PubMed:24598748}; KM=8.8 uM for L-glutaminyl 2-naphthylamide (Gln-beta-NA) (in the absence of Zn(2+), at 25 degrees Celsius) {ECO:0000269|PubMed:24598748}; Note=kcat is 8.8 sec(-1) for L-glutaminyl 2-naphthylamide (Gln-beta- NA) in the presence of Zn(2+). kcat is 6.9 sec(-1) for L-glutaminyl 2-naphthylamide (Gln-beta-NA) in the absence of Zn(2+). {ECO:0000269|PubMed:24598748}; |
| Subcellular location: | |
Secreted {ECO:0000305}. |
| Tissue specificity: | |
Detected in salivary gland and midgut. {ECO:0000269|PubMed:23770496}. |
| Induction: | |
Strongly up-regulated in salivary gland during blood-feeding on the host. Peak expression is detected 48 hours after feeding begins. {ECO:0000269|PubMed:23770496}. |
| Disruption phenotype: | |
RNAi-mediated knockdown results in reduced enzymatic activity in midgut and salivary gland tissue extracts. Weight gain after feeding is reduced. Females lay very small eggs which fail to hatch into larvae. {ECO:0000269|PubMed:23770496}. |
| Similarity: | |
Belongs to the glutaminyl-peptide cyclotransferase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.