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PDBsum entry 7cpp
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Oxidoreductase(oxygenase)
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PDB id
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7cpp
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References listed in PDB file
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Key reference
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Title
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The structural basis for substrate-Induced changes in redox potential and spin equilibrium in cytochrome p-450cam.
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Authors
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R.Raag,
T.L.Poulos.
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Ref.
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Biochemistry, 1989,
28,
917-922.
[DOI no: ]
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PubMed id
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Abstract
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The crystal structures of cytochrome P-450CAM complexed with the alternative
substrates norcamphor and adamantanone have been refined at 2.0-A resolution and
compared with the native, camphor-bound form of the enzyme. Norcamphor lacks the
8-, 9-, and 10-methyl groups of camphor. Thus, specific interactions between
these groups and phenylalanine 87 and valines 247 and 295 are missing in the
norcamphor complex. As a result, norcamphor binds about 0.9 A further from the
oxygen-binding site than does camphor, which allows sufficient room for a water
molecule or hydroxide ion to remain coordinated with the heme iron atom. The
larger adamantanone occupies a position closer to that of camphor and, as in the
camphor-bound enzyme, the heme iron remains pentacoordinate with no solvent
molecule coordinated as a sixth ligand. A comparison of crystallographic
temperature factors indicates that norcamphor is more "loosely" bound than are
either camphor or adamantanone, as might be expected from the relative sizes of
the different substrates. The looser fit of norcamphor in the active-site pocket
results in a less specific pattern of hydroxylation. The presence of an aqua
ligand is the likely structural basis for the norcamphor-P-450CAM complex having
both a lower redox potential and higher percentage of low-spin heme than do
either the camphor-P-450CAM or adamantanone-P-450CAM complexes.
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Secondary reference #1
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Title
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X-Ray crystallographic structural studies of cytochrome p-450=CAM=+
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Authors
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R.Raag,
T.L.Poulos.
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Ref.
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TO BE PUBLISHED ...
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Secondary reference #2
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Title
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Crystal structures of cytochrome p-450cam complexed with camphane, Thiocamphor, And adamantane: factors controlling p-450 substrate hydroxylation.
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Authors
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R.Raag,
T.L.Poulos.
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Ref.
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Biochemistry, 1991,
30,
2674-2684.
[DOI no: ]
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PubMed id
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Secondary reference #3
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Title
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Crystal structure of the carbon monoxide-Substrate-Cytochrome p-450cam ternary complex.
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Authors
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R.Raag,
T.L.Poulos.
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Ref.
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Biochemistry, 1989,
28,
7586-7592.
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PubMed id
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