PDBsum entry 7cep

Biosynthetic protein

PDB id: 7cep

Contents

Protein chain

414 a.a.

Ligands

PEG ×6

7TS

PGE

Waters ×247

PDB id:

7cep

Name:

Biosynthetic protein

Title:

Crystal structure of l-cycloserine-bound form of cysteine desulfurase sufs from bacillus subtilis

Structure:

Cysteine desulfurase sufs. Chain: a. Engineered: yes

Source:

Bacillus subtilis subsp. Subtilis str. 168. Organism_taxid: 224308. Strain: 168. Gene: sufs, csd, yurw, bsu32690. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008. Expression_system_variant: c41

Resolution:

2.05Å R-factor: 0.167 R-free: 0.186

Authors:

R.Nakamura,T.Yasuhiro,T.Fujishiro

Key ref:

R.Nakamura et al. Cycloserine enantiomers inhibit plp-Dependent cystein desulfurase sufs via distinct mechanisms.. Febs j, . PubMed id: 35395703

Date:

24-Jun-20 Release date: 30-Jun-21

Protein chain

O32164  (SUFS_BACSU) -  Cysteine desulfurase SufS from Bacillus subtilis (strain 168)

Seq: 406 a.a.

Struc: 414 a.a.

Enzyme reactions

• Enzyme class: E.C.2.8.1.7 - cysteine desulfurase.
• Reaction: (sulfur carrier)-H + L-cysteine = (sulfur carrier)-SH + L-alanine
• Cofactor: Pyridoxal 5'-phosphate

Pyridoxal 5'-phosphate (Bound ligand (Het Group name = 7TS) matches with 65.22% similarity)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site