PDBsum entry 7b0c

DNA binding protein

PDB id: 7b0c

Contents

Protein chains

144 a.a.

DNA/RNA

Ligands

SF4 ×2

Waters ×4

PDB id:

7b0c

Name:

DNA binding protein

Title:

[4fe-4s]-nsrr complexed to 23-bp hmpa1 operator fragment

Structure:

Hth-type transcriptional repressor nsrr. Chain: a, b. Engineered: yes. DNA (5'- d(p Ap Ap Cp Ap Cp Gp Ap Ap Tp Ap Tp Cp Ap Tp Cp Tp Ap Cp Cp Ap Ap Tp T)-3'). Chain: c. Engineered: yes. DNA (5'-

Source:

Streptomyces coelicolor a3(2). Organism_taxid: 100226. Gene: nsrr, sco7427, sc6d11.23. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Organism_taxid: 100226

Resolution:

3.00Å R-factor: 0.220 R-free: 0.236

Authors:

R.Rohac,J.C.Fontecilla-Camps,A.Volbeda

Key ref:

R.Rohac et al. Structural determinants of protein-Dna recognition in family [fes]-Transcription factor-Promoter complexes. To be published, . PubMed id: 35908109

Date:

19-Nov-20 Release date: 01-Jun-22

Protein chains

Q9L132  (NSRR_STRCO) -  HTH-type transcriptional repressor NsrR from Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)

Seq: 148 a.a.

Struc: 144 a.a.

DNA/RNA chains

A-A-C-A-C-G-A-A-T-A-T-C-A-T-C-T-A-C-C-A-A-T-T 23 bases

A-A-T-T-G-G-T-A-G-A-T-G-A-T-A-T-T-C-G-T-G-T-T 23 bases