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PDBsum entry 7a8h
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Fluorescent protein
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PDB id
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7a8h
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References listed in PDB file
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Key reference
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Title
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Structure-Function dataset reveals environment effects within a fluorescent protein model system.
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Authors
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E.De zitter,
S.Hugelier,
S.Duwé,
W.Vandenberg,
A.G.Tebo,
L.Van meervelt,
P.Dedecker.
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Ref.
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Angew Chem Int Ed Engl, 2021,
60,
10073-10081.
[DOI no: ]
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PubMed id
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Abstract
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Anisotropic environments can drastically alter the spectroscopy and
photochemistry of molecules, leading to complex structure-function
relationships. We examined this using fluorescent proteins as easy-to-modify
model systems. Starting from a single scaffold, we have developed a range of 27
photochromic fluorescent proteins that cover a broad range of spectroscopic
properties, including the determination of 43 crystal structures. Correlation
and principal component analysis confirmed the complex relationship between
structure and spectroscopy, but also allowed us to identify consistent trends
and to relate these to the spatial organization. We find that changes in
spectroscopic properties can come about through multiple underlying mechanisms,
of which polarity, hydrogen bonding and presence of water molecules are key
modulators. We anticipate that our findings and rich structure/spectroscopy
dataset can open opportunities for the development and evaluation of new and
existing protein engineering methods.
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