PDBsum entry 7xp6

Membrane protein

PDB id: 7xp6

Contents

Protein chains

240 a.a.

341 a.a.

58 a.a.

129 a.a.

283 a.a.

Ligands

SY9

PDB id:

7xp6

Name:

Membrane protein

Title:

Cryo-em structure of a class t gpcr in active state

Structure:

Guanine nucleotide-binding protein g(t) subunit alpha-3. Chain: a. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit

Source:

Homo sapiens. Organism_taxid: 9606. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Human. Gene: gnb1. Gene: gng2. Expressed in: escherichia coli. Expression_system_taxid: 562.

Authors:

Z.J.Liu,T.Hua,W.X.Xu,L.J.Wu

Key ref:

W.Xu et al. (2022). Structural basis for strychnine activation of human b taste receptor tas2r46.. Science, 377, 1298. PubMed id: 36108005 DOI: 10.1126/SCIENCE.ABO1633

Date:

03-May-22 Release date: 12-Oct-22

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 240 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 341 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 58 a.a.

Protein chain

No UniProt id for this chain

Struc: 129 a.a.

Protein chain

P16218  (GUNH_CLOTH) -  Endoglucanase H from Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372)

Seq: 900 a.a.

Struc: 283 a.a.*

Protein chain

P59540  (T2R46_HUMAN) -  Taste receptor type 2 member 46 from Homo sapiens

Seq: 309 a.a.

Struc: 283 a.a.

* PDB and UniProt seqs differ at 73 residue positions (black crosses)

Enzyme reactions

• Enzyme class 1: Chain A: E.C.3.6.5.- - ?????
• Enzyme class 2: Chain R: E.C.3.2.1.4 - cellulase.
• Reaction: Endohydrolysis of 1,4-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans.

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.