PDBsum entry 7w0n

Membrane protein

PDB id: 7w0n

Contents

Protein chains

226 a.a.

339 a.a.

61 a.a.

297 a.a.

234 a.a.

15 a.a.

PDB id:

7w0n

Name:

Membrane protein

Title:

Cryo-em structure of a dimeric gpcr-gi complex with peptide

Structure:

Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a. Synonym: adenylate cyclase-inhibiting g alpha protein. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: b. Synonym: transducin beta chain 1. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gnai1. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Gene: gnb1. Gene: gng2. Escherichia coli, homo sapiens.

Authors:

F.Xu,Y.Yue,L.J.Wu,L.E.Liu,M.Hanson

Key ref:

Y.Yue et al. (2022). Structural insight into apelin receptor-G protein stoichiometry.. Nat struct mol biol, 29, 688. PubMed id: 35817871

Date:

18-Nov-21 Release date: 27-Jul-22

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 226 a.a.

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 339 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 61 a.a.

Protein chains

P0ABE7  (C562_ECOLX) -  Soluble cytochrome b562 from Escherichia coli

Seq: 128 a.a.

Struc: 297 a.a.*

Protein chains

P35414  (APJ_HUMAN) -  Apelin receptor from Homo sapiens

Seq: 380 a.a.

Struc: 297 a.a.

Protein chain

No UniProt id for this chain

Struc: 234 a.a.

Protein chains

P0DMC3  (ELA_HUMAN) -  Apelin receptor early endogenous ligand from Homo sapiens

Seq: 54 a.a.

Struc: 15 a.a.

* PDB and UniProt seqs differ at 70 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????