PDBsum entry 7t96

Membrane protein

PDB id: 7t96

Contents

Protein chains

274 a.a.

218 a.a.

338 a.a.

56 a.a.

236 a.a.

Ligands

ACH

2CU

PDB id:

7t96

Name:

Membrane protein

Title:

Cryo-em structure of s2 state ach-bound m2r-go signaling complex with a pam

Structure:

Muscarinic acetylcholine receptor m2. Chain: a. Engineered: yes. Guanine nucleotide-binding protein g(o) subunit alpha. Chain: b. Engineered: yes. Guanine nucleotide-binding protein g(i)/g(s)/g(t) subunit beta-1. Chain: c.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: chrm2. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gnao1. Expressed in: trichoplusia ni. Expression_system_taxid: 7111.

Authors:

J.Xu,Q.Wang,Y.Du,B.K.Kobilka

Key ref:

J.Xu et al. Cryo-Em structure of s2 state ach-Bound m2r-Go signal complex with a pam. To be published, . PubMed id: 36690613

Date:

18-Dec-21 Release date: 25-Jan-23

Protein chain

P08172  (ACM2_HUMAN) -  Muscarinic acetylcholine receptor M2 from Homo sapiens

Seq: 466 a.a.

Struc: 274 a.a.

Protein chain

P09471  (GNAO_HUMAN) -  Guanine nucleotide-binding protein G(o) subunit alpha from Homo sapiens

Seq: 354 a.a.

Struc: 218 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 338 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 56 a.a.

Protein chain

No UniProt id for this chain

Struc: 236 a.a.

* PDB and UniProt seqs differ at 4 residue positions (black crosses)