PDBsum entry 7t10

Membrane protein/signaling protein

PDB id: 7t10

Contents

Protein chains

287 a.a.

218 a.a.

338 a.a.

57 a.a.

14 a.a.

232 a.a.

Waters ×5

PDB id:

7t10

Name:

Membrane protein/signaling protein

Title:

Cryoem structure of somatostatin receptor 2 in complex with somatostatin-14 and gi3

Structure:

Somatostatin receptor type 2,kappa-type opioid receptor. Chain: r. Engineered: yes. Other_details: somatostatin receptor type 2 with intracellular loop 3 (icl3) replaced with the icl3 from the kappa-type opioid receptor. Guanine nucleotide-binding protein g(i) subunit alpha-3. Chain: a. Synonym: g(i) alpha-3. Engineered: yes.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: sstr2, oprk1, oprk. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Gene: gnai3. Expressed in: trichoplusia ni. Expression_system_taxid: 7111.

Authors:

M.J.Robertson,G.Skinotis

Key ref:

M.J.Robertson et al. Plasticity in ligand recognition at somatostatin rece. Nat struct mol biol, . PubMed id: 35210615

Date:

30-Nov-21 Release date: 09-Mar-22

Protein chain

P30874  (SSR2_HUMAN) -  Somatostatin receptor type 2 from Homo sapiens

Seq: 369 a.a.

Struc: 287 a.a.*

Protein chain

P41145  (OPRK_HUMAN) -  Kappa-type opioid receptor from Homo sapiens

Seq: 380 a.a.

Struc: 287 a.a.*

Protein chain

P08754  (GNAI3_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-3 from Homo sapiens

Seq: 354 a.a.

Struc: 218 a.a.*

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 338 a.a.

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 57 a.a.

Protein chain

P61278  (SMS_HUMAN) -  Somatostatin from Homo sapiens

Seq: 116 a.a.

Struc: 14 a.a.

Protein chain

No UniProt id for this chain

Struc: 232 a.a.

* PDB and UniProt seqs differ at 175 residue positions (black crosses)