PDBsum entry 7piu

Signaling protein

PDB id: 7piu

Contents

Protein chains

259 a.a.

189 a.a.

337 a.a.

55 a.a.

128 a.a.

Ligands

ARG-CYS-DAL-HIS-DPN-ARG-TRP-CYS

Metals

_CA

Waters ×91

PDB id:

7piu

Name:

Signaling protein

Title:

Cryo-em structure of the agonist setmelanotide bound to the active melanocortin-4 receptor (mc4r) in complex with the heterotrimeric gs protein at 2.6 a resolution.

Structure:

Melanocortin receptor 4. Chain: r. Synonym: mc4-r. Engineered: yes. Setmelanotide (other names rm-493. Bim-22493. Irc-022493. Imcivree). Chain: p. Engineered: yes. Isoform gnas-2 of guanine nucleotide-binding protein g(s)

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mc4r. Expressed in: spodoptera aff. Frugiperda 1 bold-2017. Expression_system_taxid: 2449148. Synthetic: yes. Synthetic construct. Organism_taxid: 32630.

Authors:

N.A.Heyder,A.Schmidt,G.Kleinau,T.Hilal,P.Scheerer

Key ref:

N.A.Heyder et al. (2021). Structures of active melanocortin-4 receptor-Gs-Prote complexes with ndp-Alpha-Msh and setmelanotide.. Cell res, 31, 1176. PubMed id: 34561620

Date:

23-Aug-21 Release date: 17-Nov-21

Protein chain

P32245  (MC4R_HUMAN) -  Melanocortin receptor 4 from Homo sapiens

Seq: 332 a.a.

Struc: 259 a.a.

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 189 a.a.*

Protein chain

P54311  (GBB1_RAT) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Rattus norvegicus

Seq: 340 a.a.

Struc: 337 a.a.

Protein chain

P63212  (GBG2_BOVIN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Bos taurus

Seq: 71 a.a.

Struc: 55 a.a.

Protein chain

No UniProt id for this chain

Struc: 128 a.a.

* PDB and UniProt seqs differ at 28 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????