PDBsum entry 7f4d

Membrane protein

PDB id: 7f4d

Contents

Protein chains

56 a.a.

234 a.a.

127 a.a.

338 a.a.

263 a.a.

12 a.a.

Metals

_CA

PDB id:

7f4d

Name:

Membrane protein

Title:

Cryo-em structure of alpha-msh-bound melanocortin-1 receptor in complex with gs protein and nb35

Structure:

Guanine nucleotide-binding protein g(i)/g(s)/g(o) subunit gamma-2. Chain: g. Synonym: g gamma-i. Engineered: yes. Guanine nucleotide-binding protein g(i) subunit alpha-1. Chain: a. Engineered: yes. Nanobody 35.

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: gng2. Expressed in: trichoplusia ni. Expression_system_taxid: 7111. Gene: gnas, gnas1, gsp, gnai1. Synthetic construct. Organism_taxid: 32630.

Authors:

S.Ma,Y.Chen,A.Dai,W.Yin,J.Guo,D.Yang,F.Zhou,Y.Jiang,M.-W.Wang,H.E.Xu

Key ref:

S.Ma et al. (2021). Structural mechanism of calcium-Mediated hormone reco and g beta interaction by the human melanocortin-1 re. Cell res, 31, 1061. PubMed id: 34453129

Date:

18-Jun-21 Release date: 08-Sep-21

Protein chain

P59768  (GBG2_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 from Homo sapiens

Seq: 71 a.a.

Struc: 56 a.a.

Protein chain

P63092  (GNAS2_HUMAN) -  Guanine nucleotide-binding protein G(s) subunit alpha isoforms short from Homo sapiens

Seq: 394 a.a.

Struc: 234 a.a.*

Protein chain

P63096  (GNAI1_HUMAN) -  Guanine nucleotide-binding protein G(i) subunit alpha-1 from Homo sapiens

Seq: 354 a.a.

Struc: 234 a.a.*

Protein chain

No UniProt id for this chain

Struc: 127 a.a.

Protein chain

P62873  (GBB1_HUMAN) -  Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 from Homo sapiens

Seq: 340 a.a.

Struc: 338 a.a.

Protein chain

P0ABE7  (C562_ECOLX) -  Soluble cytochrome b562 from Escherichia coli

Seq: 128 a.a.

Struc: 263 a.a.*

Protein chain

Q01726  (MSHR_HUMAN) -  Melanocyte-stimulating hormone receptor from Homo sapiens

Seq: 317 a.a.

Struc: 263 a.a.

Protein chain

No UniProt id for this chain

Struc: 12 a.a.

* PDB and UniProt seqs differ at 312 residue positions (black crosses)

Enzyme reactions

• Enzyme class: Chain A: E.C.3.6.5.- - ?????