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PDBsum entry 6zfc
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Sugar binding protein
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PDB id
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6zfc
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References listed in PDB file
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Key reference
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Title
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Fucosylated ubiquitin and orthogonally glycosylated mutant a28c: conceptually new ligands for burkholderia ambifaria lectin (bambl).
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Authors
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S.Kuhaudomlarp,
L.Cerofolini,
S.Santarsia,
E.Gillon,
S.Fallarini,
G.Lombardi,
M.Denis,
S.Giuntini,
C.Valori,
M.Fragai,
A.Imberty,
A.Dondoni,
C.Nativi.
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Ref.
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Chem Sci, 2020,
11,
12662-12670.
[DOI no: ]
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PubMed id
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Abstract
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Two orthogonal, metal free click reactions, enabled to glycosylate ubiquitin and
its mutant A28C forming two protein scaffolds with high affinity for BambL, a
lectin from the human pathogen Burkholderia ambifaria. A new fucoside
analogue, with high affinity with BambL, firstly synthetized and co-crystallized
with the protein target, provided the insights for sugar determinants grafting
onto ubiquitin. Three ubiquitin-based glycosides were thus assembled.
Fuc-Ub, presented several copies of the fucoside analogue, with proper
geometry for multivalent effect; Rha-A28C, displayed one thio-rhamnose,
known for its ability to tuning the immunological response; finally,
Fuc-Rha-A28C, included both multiple fucoside analogs and the rhamnose
residue. Fuc-Ub and Fuc-Rha-A28C ligands proved high affinity for
BambL and unprecedented immune modulatory properties towards macrophages
activation.
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