UniProt functional annotation for A0A0S2UQQ5



	UniProt functional annotation for A0A0S2UQQ5

UniProt code: A0A0S2UQQ5.

Organism: Paenibacillus barcinonensis.

Taxonomy: Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.

Function: Involved in depolymerization of xylan, a major component of the lignocellulosic substrates. Acts as an exo-oligoxylanase that efficiently hydrolyzes xylooligosaccharides, releasing xylose from their reducing ends. Hydrolyzes xylooligomers of 3 to 6 xylose units to xylose and xylobiose. Besides linear xylooligosaccharides, also hydrolyzes branched xylooligomers, such as xylooligomers decorated with 4-O-methyl-D-glucuronic acid moieties. Its proposed role is the degradation of xylooligomers produced by the activity of extracellular xylanases once they have been transported inside cells. Shows minor activity on polymeric xylan (glucuronoxylan from beechwood). Is not active on cellooligosaccharides or cellulosic substrates, or on other polysaccharides such as pectin, polygalacturonic acid, laminarin, or lichenan. {ECO:0000269|PubMed:27316951}.

Catalytic activity: Reaction=Hydrolysis of (1->4)-beta-D-xylose residues from the reducing end of oligosaccharides.; EC=3.2.1.156; Evidence={ECO:0000269|PubMed:27316951};

Biophysicochemical properties: Kinetic parameters: KM=1.64 mM for xylotriose {ECO:0000269|PubMed:27316951}; Vmax=152.2 umol/min/mg enzyme for the hydrolysis of xylotriose {ECO:0000269|PubMed:27316951}; Note=kcat is 118.8 sec(-1) for the hydrolysis of xylotriose. {ECO:0000269|PubMed:27316951}; pH dependence: Optimum pH is 7 for the hydrolysis of xylotriose. {ECO:0000269|PubMed:27316951}; Temperature dependence: Optimum temperature is 40 degrees Celsius for the hydrolysis of xylotriose. {ECO:0000269|PubMed:27316951};

Pathway: Glycan degradation; xylan degradation. {ECO:0000269|PubMed:27316951}.

Similarity: Belongs to the glycosyl hydrolase 8 (cellulase D) family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Paenibacillus barcinonensis.
Taxonomy:		Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.



Function:		Involved in depolymerization of xylan, a major component of the lignocellulosic substrates. Acts as an exo-oligoxylanase that efficiently hydrolyzes xylooligosaccharides, releasing xylose from their reducing ends. Hydrolyzes xylooligomers of 3 to 6 xylose units to xylose and xylobiose. Besides linear xylooligosaccharides, also hydrolyzes branched xylooligomers, such as xylooligomers decorated with 4-O-methyl-D-glucuronic acid moieties. Its proposed role is the degradation of xylooligomers produced by the activity of extracellular xylanases once they have been transported inside cells. Shows minor activity on polymeric xylan (glucuronoxylan from beechwood). Is not active on cellooligosaccharides or cellulosic substrates, or on other polysaccharides such as pectin, polygalacturonic acid, laminarin, or lichenan. {ECO:0000269\|PubMed:27316951}.


Catalytic activity:		Reaction=Hydrolysis of (1->4)-beta-D-xylose residues from the reducing end of oligosaccharides.; EC=3.2.1.156; Evidence={ECO:0000269\|PubMed:27316951};
Biophysicochemical properties:		Kinetic parameters: KM=1.64 mM for xylotriose {ECO:0000269\|PubMed:27316951}; Vmax=152.2 umol/min/mg enzyme for the hydrolysis of xylotriose {ECO:0000269\|PubMed:27316951}; Note=kcat is 118.8 sec(-1) for the hydrolysis of xylotriose. {ECO:0000269\|PubMed:27316951}; pH dependence: Optimum pH is 7 for the hydrolysis of xylotriose. {ECO:0000269\|PubMed:27316951}; Temperature dependence: Optimum temperature is 40 degrees Celsius for the hydrolysis of xylotriose. {ECO:0000269\|PubMed:27316951};
Pathway:		Glycan degradation; xylan degradation. {ECO:0000269\|PubMed:27316951}.
Similarity:		Belongs to the glycosyl hydrolase 8 (cellulase D) family. {ECO:0000305}.