 |
PDBsum entry 6t90
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Transcription
|
PDB id
|
|
|
|
6t90
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
94 a.a.
|
|
|
|
|
|
|
|
|
|
|
82 a.a.
|
|
|
|
|
|
|
|
|
|
|
109 a.a.
|
|
|
|
|
|
|
|
|
|
|
93 a.a.
|
|
|
|
|
|
|
|
|
|
|
73 a.a.
|
|
|
|
|
|
|
|
|
|
|
74 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Mechanisms of oct4-Sox2 motif readout on nucleosomes.
|
|
|
Authors
|
|
A.K.Michael,
R.S.Grand,
L.Isbel,
S.Cavadini,
Z.Kozicka,
G.Kempf,
R.D.Bunker,
A.D.Schenk,
A.Graff-Meyer,
G.R.Pathare,
J.Weiss,
S.Matsumoto,
L.Burger,
D.Schübeler,
N.H.Thomä.
|
|
|
Ref.
|
|
Science, 2020,
368,
1460-1465.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Transcription factors (TFs) regulate gene expression through chromatin where
nucleosomes restrict DNA access. To study how TFs bind nucleosome-occupied
motifs, we focused on the reprogramming factors OCT4 and SOX2 in mouse embryonic
stem cells. We determined TF engagement throughout a nucleosome at base-pair
resolution in vitro, enabling structure determination by cryo-electron
microscopy at two preferred positions. Depending on motif location, OCT4 and
SOX2 differentially distort nucleosomal DNA. At one position, OCT4-SOX2 removes
DNA from histone H2A and histone H3; however, at an inverted motif, the TFs only
induce local DNA distortions. OCT4 uses one of its two DNA-binding domains to
engage DNA in both structures, reading out a partial motif. These findings
explain site-specific nucleosome engagement by the pluripotency factors OCT4 and
SOX2, and they reveal how TFs distort nucleosomes to access chromatinized motifs.
|
|
|
|
|
|
|
