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PDBsum entry 6s2c
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References listed in PDB file
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Key reference
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Title
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Acquired functional capsid structures in metazoan totivirus-Like dsrna virus.
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Authors
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K.Okamoto,
R.J.Ferreira,
D.S.D.Larsson,
F.R.N.C.Maia,
H.Isawa,
K.Sawabe,
K.Murata,
J.Hajdu,
K.Iwasaki,
P.M.Kasson,
N.Miyazaki.
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Ref.
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Structure, 2020,
28,
888.
[DOI no: ]
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PubMed id
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Abstract
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Non-enveloped icosahedral double-stranded RNA (dsRNA) viruses possess
multifunctional capsids required for their proliferation. Whereas
protozoan/fungal dsRNA viruses have a relatively simple capsid structure, which
suffices for the intracellular phase in their life cycle, metazoan dsRNA viruses
have acquired additional structural features as an adaptation for extracellular
cell-to-cell transmission in multicellular hosts. Here, we present the first
atomic model of a metazoan dsRNA totivirus-like virus and the structure reveals
three unique structural traits: a C-terminal interlocking arm, surface
projecting loops, and an obstruction at the pore on the 5-fold symmetry axis.
These traits are keys to understanding the capsid functions of metazoan dsRNA
viruses, such as particle stability and formation, cell entry, and endogenous
intraparticle transcription of mRNA. On the basis of molecular dynamics
simulations of the obstructed pore, we propose a possible mechanism of
intraparticle transcription in totivirus-like viruses, which dynamically
switches between open and closed states of the pore(s).
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